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enzymatic hydrolysis of cellulosic materials using simultaneous ball milling

enzymatic hydrolysis of cellulosic materials using simultaneous ball milling

Aball-mill reactorwas used as the digestion vessel, and the extent and rate ofhydrolysiswere monitored. Concurrently,enzyme adsorptionprofiles and the rate of conversion during the course ofhydrolysiswere monitored.alpha-Cellulose, employed as a model substrate, and SO2-impregnated steam-exploded Douglas-fir wood chips were assessed as thecellulosic substrates.

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  • Enhancing the enzymatic hydrolysis of cellulosic materials
    Enhancing the enzymatic hydrolysis of cellulosic materials

    Mar 01, 2002· It was apparent that the combined strategy ofsimultaneous ball millingand enzymatic hydrolysis could improve the rate of saccharification and/or reduce theenzyme loadingrequired to attain totalhydrolysisof thecarbohydrate moieties. One of the limiting factors restricting the effective and efficient bioconversion of softwood-derived lignocellulosic residues is the recalcitrance of the.

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  • Enhancing the Enzymatic Hydrolysis of Cellulosic Materials
    Enhancing the Enzymatic Hydrolysis of Cellulosic Materials

    α-cellulose saccharification using simultaneous ball millingand enzymatic hydrolysis at 2.5% (w/v) and 25 FPU/g ofcellulose.Cellulose Hydrolysisin a Ball Mill Reactor

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  • Enhancing the Enzymatic Hydrolysis of Cellulosic Materials
    Enhancing the Enzymatic Hydrolysis of Cellulosic Materials

    Aball-mill reactorwasusedas the digestion vessel, and the extent and rate ofhydrolysiswere monitored. Concurrently,enzymeadsorption profiles and the rate of conversion during the course ofhydrolysiswere monitored.α-Cellulose, employed as a model substrate, and SO 2-impregnated steam-exploded Douglas-fir wood chips were assessed as thecellulosicsubstrates. The softwood-derived …

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  • Enzymatic Hydrolysis Of Cellulosic Materials Using
    Enzymatic Hydrolysis Of Cellulosic Materials Using

    Theuse of ball millingtoobtain cellulose nanocrystalswas also explored by the group of Yu who extracted CNCs from ballmilled wood via a multistep process 40 DouglasFir wood chips were firstly hammermilled to wood flours which were then treatedusingaplanetary ball millat 270 rpm This process was followed byenzymatic hydrolysis.

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  • Application of intermittent ball milling to enzymatic
    Application of intermittent ball milling to enzymatic

    Hydrolysisof lignocellulosic biomass is important for isolation of glucose in a biorefinery. In this research, intermittentball millingwas applied to facilitate and enhanceenzymatic hydrolysisof dilute acid-pretreated lignocellulosic biomass, with the highest glucose yield of 66.5% at a lowenzymedose (10 FPU g −1 glucan) over 24h. In comparison, the yield for the typical liquid-state ...

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  • Fermentation with continuous ball milling Effectiveness
    Fermentation with continuous ball milling Effectiveness

    Mar 01, 2020·Millingas a pretreatment prior tohydrolysis usingfungal cellulase has been investigated in several studies [, , ] but energy requirements are widely thought to be too high to be practical [, , ]. However, the physical propertiesof cellulosicbiomass slurries undergo large changes during biologically-mediated solubilization.

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  • Cellulose emulsions and their hydrolysis Alfassi 2019
    Cellulose emulsions and their hydrolysis Alfassi 2019

    The full text of this article hosted at iucr.org is unavailable due to technical difficulties.

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  • Enhancementof celluloseaccessibility andenzymatic
    Enhancementof celluloseaccessibility andenzymatic

    @article{osti_5066592, title = {Enhancementof celluloseaccessibility andenzymatic hydrolysisbysimultaneouswetmilling}, author = {Kelsey, R G and Shafizadeh, F}, abstractNote = {It has been shown that the rate ofenzymaticsaccharificationof cellulosic materialsincluding purecellulose, newsprint, lignocellulose and wood can be substantially increased bysimultaneouswetmilling.

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  • Ball milling a green technology for the preparation and
    Ball milling a green technology for the preparation and

    Theuseofball millingto obtaincellulosenanocrystals was also explored by the group of Yu, who extracted CNCs fromball-milled wood via a multi-step process. 40 Douglas-Fir wood chips were firstly hammer-milled to wood flours, which were then treatedusinga planetaryball millat 270 rpm. This process was followed byenzymatic hydrolysis...

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  • High solidsenzymatic hydrolysisofball milled corn
    High solidsenzymatic hydrolysisofball milled corn

    Apr 20, 2020· High-solidsenzymatic hydrolysishas attracted increasing attentions for the production of bioethanol from lignocellulosic biomass with its advantages of high product concentration, water saving, and low energy and capital costs. However, the increase of solids content would worsen the rheological properties, resulting in heat/mass transfer limitation and higher mixing energy.

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  • Enhancementof celluloseaccessibility andenzymatic
    Enhancementof celluloseaccessibility andenzymatic

    10.1002/bit.260220511.abs It has been shown that the rate ofenzymaticsaccharificationof cellulosic materialsincluding “pure”cellulose(Whatman CF−11cellulose), newsprint, lignocellulose (prehydrolyzed to remove hemicelluloses), and wood can be substantially increased bysimultaneouswetmilling. An enhancedhydrolysisrate was sustained above that observed forball milling...

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  • Energy requirements and process design considerations in
    Energy requirements and process design considerations in

    Theenzymatic hydrolysisyield was found to be directly related to the specific energy input to thecellulosicsubstrate (kWh/1b substrate) by compressionmilling, and the energy input can be controlled by themillingtime. Theenzymatic hydrolysisyeilds fromcellulosic materialspretreated by compressionmillingalso vary significantly ...

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  • EfficientEnzymatic Hydrolysisof Biomass Hemicellulose in
    EfficientEnzymatic Hydrolysisof Biomass Hemicellulose in

    Currentenzymaticmethods for hemicellulosic biomass depolymerization are solution-based, typically require a harsh chemical pre-treatment of thematerialand large volumes of water, yet lack in efficiency. In our study, xylanase (E.C. 3.2.1.8) from Thermomyces lanuginosus is used to hydrolyze xylans from different sources. We report an innovativeenzymaticprocess which avoids theuseof bulk ...

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  • Effects of Crystallinity on Dilute AcidHydrolysisof
    Effects of Crystallinity on Dilute AcidHydrolysisof

    The dilute acid (0.05 M H2SO4)hydrolysisat 175 °C of samples comprised of varying fractions of crystalline (α-form) and amorphouscellulosewas studied. The amorphous content, based on XRD and CP/MAS NMR, and the product (glucose) yield, based on HPLC, increased by as much as a factor of 3 uponball milling. These results are interpreted in terms of a model involving mechanical disruption ...

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  • Toward an aggregated understanding ofenzymatic hydrolysis
    Toward an aggregated understanding ofenzymatic hydrolysis

    Nov 10, 2004· Drake Mboowa, Vinay Khatri, Jack N. Saddler, Theuseof fluorescent protein-tagged carbohydrate-binding modules to evaluate the influence of drying oncelluloseaccessibility andenzymatic hydrolysis, RSC Advances, 10.1039/D0RA05333C, 10, 45, (27152-27160), (2020).

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  • Combining hot compressed water andball milling
    Combining hot compressed water andball milling

    Background. Lignocellulosic biomass such as wood is an attractivematerialfor fuel ethanol production. Pretreatment technologies that increase the digestibilityof celluloseand hemicellulose in the lignocellulosic biomass have a major influence on the cost of the subsequentenzymatic hydrolysisand ethanol fermentation processes.

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  • Acidhydrolysisand kinetics of bioethanol production from
    Acidhydrolysisand kinetics of bioethanol production from

    May 01, 2009· A study was conductedusingaball millreactor for the pre-treatment andhydrolysisof acellulose and S[O.sub.2] impregnated steam-exploded Douglas fir wood chips (17). They reported the number ofballbeads as an effective parameter to improveenzymatic hydrolysisof [alpha]-cellulose.

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  • Ball milling a green technology for the preparation and
    Ball milling a green technology for the preparation and

    Theuseofball millingto obtaincellulosenanocrystals was also explored by the group of Yu, who extracted CNCs fromball-milled wood via a multi-step process. 40 Douglas-Fir wood chips were firstly hammer-milled to wood flours, which were then treatedusinga planetaryball millat 270 rpm. This process was followed byenzymatic hydrolysis...

    Get Price
  • Enhancementof celluloseaccessibility andenzymatic
    Enhancementof celluloseaccessibility andenzymatic

    10.1002/bit.260220511.abs It has been shown that the rate ofenzymaticsaccharificationof cellulosic materialsincluding “pure”cellulose(Whatman CF−11cellulose), newsprint, lignocellulose (prehydrolyzed to remove hemicelluloses), and wood can be substantially increased bysimultaneouswetmilling. An enhancedhydrolysisrate was sustained above that observed forball milling...

    Get Price
  • Study of Chemical andEnzymatic Hydrolysis of Cellulosic
    Study of Chemical andEnzymatic Hydrolysis of Cellulosic

    The objective of this study was to evaluate the chemical andenzymatic hydrolysis usinga factorial experimental design (2 3 ) in order to obtain fermentable sugars fromcellulose-basedmaterial(CBM) usually used as pet litter. In assessing chemicalhydrolysis, we studied the effect of temperature, in addition to H 2 SO 4 concentration and reaction time, on the production of total ...

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  • Comparison of Pretreatments for Lignocellulosic Biomass
    Comparison of Pretreatments for Lignocellulosic Biomass

    [4] Mais U, Esteghlalian , Saddler J N, et al. Enhancing theenzymatic hydrolysis of cellulosic materials using simultaneous ball milling[J]. Applied biochemistry and biotechnology, 2002, 98: 815-832.

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  • Pretreatment Technologies of LignocellulosicMaterialsin
    Pretreatment Technologies of LignocellulosicMaterialsin

    Enhancing theenzymatic hydrolysis of cellulosic materials using simultaneous ball milling. Applied biochemistry and biotechnology, 98(1): 815-832. Mäki-Arvela P, Anugwom I, Virtanen P, Sjöholm R, dan Mikkola JP. 2010. Dissolution of lignocellulosicmaterialsand its constituentsusing…

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  • Effects of Crystallinity on Dilute AcidHydrolysisof
    Effects of Crystallinity on Dilute AcidHydrolysisof

    The dilute acid (0.05 M H2SO4)hydrolysisat 175 °C of samples comprised of varying fractions of crystalline (α-form) and amorphouscellulosewas studied. The amorphous content, based on XRD and CP/MAS NMR, and the product (glucose) yield, based on HPLC, increased by as much as a factor of 3 uponball milling. These results are interpreted in terms of a model involving mechanical disruption ...

    Get Price
  • Effectsof CelluloseCrystallinity, Hemicellulose, and
    Effectsof CelluloseCrystallinity, Hemicellulose, and

    The effectsof cellulosecrystallinity, hemicellulose, and lignin on theenzymatic hydrolysisof Miscanthus sinensis to monosaccharides were investigated. A air-dried biomass was ground byball-milling, and the powder was separated into four fractions by passage through a series of sieves with mesh sizes 250–355 μm, 150–250 μm, 63–150 μm, and <63 μm.

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  • Effect of mixing onenzymatic hydrolysisof steam
    Effect of mixing onenzymatic hydrolysisof steam

    Enhancing theenzymatic hydrolysis of cellulosic materials using simultaneous ball milling. Appl Biochem Biotechnol. 2002; 98 :815–832. doi: 10.1385/ABAB:98-100:1-9:815.

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  • Jack Saddler Faculty Member Profile UBC Forestry
    Jack Saddler Faculty Member Profile UBC Forestry

    Effect of acetyl groups onenzymatic hydrolysis of cellulosicsubstates-Holzforschung, 94, 851-861 Berlin, A ... Fungalenzymetreatment of newsprintmillwhite water – impact on water and fiber properties. ... Enhancing theenzymatic hydrolysis of cellulosic materials using simultaneous ball milling. Applied Biochemistry and BiotechnologyVol ...

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  • Pretreatment Empty Fruit Bunch of Oil Palm Tree for
    Pretreatment Empty Fruit Bunch of Oil Palm Tree for

    Jul 24, 2017· Empty fruit bunch of oil palm tree (EFBOPT), solid waste of palm oil industries, is potential for rawmaterialsof biofuel especially bioethanol production because of itscelluloseand hemicellulose contents. There are four steps to produce bioethanol, called the second generation bioethanol, from EFBOPT or other lignocellulosicmaterials. The steps are (a) pretreatment of lignocellulose ...

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